AVC Lobster Science Centre June 6, 2008 J. Euk. Microbial., 43(4), 1996, pp.341-346
The Lobster Parasite Anophryoides haemophila (Scuticociliatida: Orchitophryidae): Nuclear 18S rDNA Sequence, Phylogeny and Detection Using Oligonucleotide Primers
Mark A. Ragan, Richard J. Cawthorn, Beatrice Depres, Colleen A. Murphy, Rama K. Singh, Micahel B. Loughlin and Robert C.
Bayer Institute for Marine Biosciences, National Research Council of Canada, 1411 Oxford Street, Halifax, Nova Acotia, Canada B3H 3Z1, Atlantic Veterinary College, University of Prince Edward Island, Canada, C1A 4P3, and Department of Animal, Veterinary and Aquatic Sciences, University of Maine, Orono, Maine 04469-5735, USA
Abstract
We have determined the nucleotide sequence of the nuclear gene encoding small-submit ribosomal ribonucleic acid of the ciliate. Anophryoides haemophila, a parasit of the American lobster Homarus Americanus. The gene is 1763 bp in length, and has a guanosine-plus-cytosine content of 43.9%. Inferred phylogenetic frameworks strongly support the monophyly of the scuticociliates, and suggest that order Scuticociliatida should be elevated to at least subclass rank. Oligonucleotide probes based on A. haemophila ssurDNA can discriminate between DNAs of A. haemophila and other investigated hymenostome ciliates, and effectively prime polymerase chain reaction-based detection of A. haemophila deoxyribonucleic acid against at least a 1600-fold excess of total deoxyribonucleic acid from H. americanus.
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